Cloned (Comment) | Organism |
---|---|
MEKK2, DNA and amino acid sequence determination, expression of wild-type and mutant MEKK2 by reticulocyte lysate TnT T7 mixture, expression of wild-type and mutant MEKK2 and MEKK1 in COS-1 cells and in HeLa cells, coexpression with ERK5 in COS-1 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
F1443A | site-directed mutagenesis of MEKK1, the mutation abolishes the in vitro interaction with MKK4, but retains the in vivo activity | Mus musculus |
F571A | site-directed mutagenesis of MEKK2, inactive mutant | Mus musculus |
I1445A | site-directed mutagenesis of MEKK1, the mutation has a deleterious effect on MEKK1 function | Mus musculus |
I1454A | site-directed mutagenesis of MEKK1, the mutation abolishes the in vitro interaction with MKK4, but retains the in vivo activity | Mus musculus |
I573A | site-directed mutagenesis of MEKK2, inactive mutant | Mus musculus |
L1458A | site-directed mutagenesis of MEKK1, the mutation has a deleterious effect on MEKK1 function | Mus musculus |
L582A/P583A | site-directed mutagenesis of MEKK2, inactive mutant | Mus musculus |
additional information | mutations in the MEKK subdomain X differentially affect MEKK2 and MEKK1 activity, overview | Mus musculus |
P1452A | site-directed mutagenesis of MEKK1, the mutation has a deleterious effect on MEKK1 function | Mus musculus |
P1455A | site-directed mutagenesis of MEKK1, the mutation has a deleterious effect on MEKK1 function | Mus musculus |
P580A | site-directed mutagenesis of MEKK2, the mutant shows 82% of wild-type activity | Mus musculus |
P584A | site-directed mutagenesis of MEKK2, the mutant shows activity similar to the wild-type enzyme | Mus musculus |
S1459A | site-directed mutagenesis of MEKK1, the mutation abolishes the in vitro interaction with MKK4, but retains the in vivo activity | Mus musculus |
T575A/Q576A/P577A | site-directed mutagenesis of MEKK2, inactive mutant | Mus musculus |
V586A | site-directed mutagenesis of MEKK2, the mutant shows activity similar to the wild-type enzyme | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MEK5 | Mus musculus | activated MEK5 activates ERK5 | ADP + phosphorylated MEK5 | - |
? | |
ATP + MKK4 | Mus musculus | a MAPKK | ADP + phosphorylated MKK4 | - |
? | |
additional information | Mus musculus | MEKK2 induces the JNK signaling pathway, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MEK5 | a MAPKK | Mus musculus | ADP + phosphorylated MEK5 | - |
? | |
ATP + MEK5 | activated MEK5 activates ERK5 | Mus musculus | ADP + phosphorylated MEK5 | - |
? | |
ATP + MKK4 | a MAPKK | Mus musculus | ADP + phosphorylated MKK4 | - |
? | |
additional information | MEKK2 induces the JNK signaling pathway, overview | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the subdomain X is required for phosphorylation and activation of MAPKKs in vivo | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
MEKK1 | - |
Mus musculus |
MEKK2 | - |
Mus musculus |
MEKK3 | - |
Mus musculus |
MEKK4 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |